SPORE GERMINATION AND SPOROGENESIS IN BACILLUS MEGATERIUM
نویسندگان
چکیده
منابع مشابه
Spore germination of Bacillus megaterium QM B1551 mutants.
Auxotrophic or antibiotic-resistant mutants of Bacillus megaterium QM B1551 are capable of initiation of germination similar to the parental (QM B1551) prototrophic strain.
متن کاملBacillus megaterium spore germination is influenced by inoculum size.
AIMS The effect of spore density on the germination (time-to-germination, percent germination) of Bacillus megaterium spores on tryptic soy agar was determined using direct microscopic observation. METHODS AND RESULTS Inoculum size varied from approximately 10(3) to 10(8) cfu ml(-1) in a medium where pH=7 and the sodium chloride concentration was 0.5% w/v. Inoculum size was measured by global...
متن کاملSpore germination mediated by Bacillus megaterium QM B1551 SleL and YpeB.
Previous work demonstrated that Bacillus megaterium QM B1551 spores that are null for the sleB and cwlJ genes, which encode cortex-lytic enzymes (CLEs), either of which is required for efficient cortex hydrolysis in Bacillus spores, could germinate efficiently when complemented with a plasmid-borne copy of ypeB plus the nonlytic portion of sleB encoding the N-terminal domain of SleB (sleB(N)). ...
متن کاملZinc release and the sequence of biochemical events during triggering of Bacillus megaterium KM spore germination.
Zinc release is the first quantitatively significant event detected during the triggering of Bacillus megaterium KM spore germination. Of the total spore Zn2+ pool 25% is released from non-heat-activated spores within 4 min of triggering germination. During this period only 10% of the spore population becomes irreversibly committed to germinate. The investigation of a putative role for Zn2+ in ...
متن کاملMetabolic requirements for microcycle sporogenesis of Bacillus megaterium.
Spores of Bacillus megaterium QM B1551 germinated, elongated, and resporulated (microcycle sporogenesis) in simple chemically defined media which permitted no cell division. The second-stage spores thus produced were heat-stable and required heat activation for germination. The original amount of spore deoxyribonucleic acid tripled before completion of the cycle. Acetate and a small amount of a...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1958
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.75.1.118-119.1958